A gene encoding SMALL ACIDIC PROTEIN 2 potentially mediates the response to synthetic auxin, 2,4-dichlorophenoxyacetic acid, in Arabidopsis thaliana

Akari Nakasone; Maki Kawai-Yamada; Tomohiro Kiyosue; Issay Narumi; Hirofumi Uchimiya; Yutaka Oono

doi:10.1016/j.jplph.2009.02.005

A gene encoding SMALL ACIDIC PROTEIN 2 potentially mediates the response to synthetic auxin, 2,4-dichlorophenoxyacetic acid, in Arabidopsis thaliana

J Plant Physiol. 2009 Aug 15;166(12):1307-1313. doi: 10.1016/j.jplph.2009.02.005. Epub 2009 Mar 23.

Authors

Akari Nakasone¹, Maki Kawai-Yamada², Tomohiro Kiyosue³, Issay Narumi⁴, Hirofumi Uchimiya⁵, Yutaka Oono⁴

Affiliations

¹ Radiation-Applied Biology Division, Japan Atomic Energy Agency, Takasaki 370-1292, Japan; Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan. Electronic address: nakasone.akari@jaea.go.jp.
² Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan; Department of Environmental Science & Technology, Saitama University, Saitama 338-0825, Japan; Japan Science and Technology Agency, Core Research for Environmental Science and Technology, Kawaguchi 332-0012, Japan.
³ Divison of Genome Analysis and Genetic Research, Life Science Research Center, Institute of Research Promotion, Kagawa University, Kagawa 761-0795, Japan.
⁴ Radiation-Applied Biology Division, Japan Atomic Energy Agency, Takasaki 370-1292, Japan.
⁵ Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan; Iwate Biotechnology Research Center, Kitakami 024-0003, Japan.

PMID: 19307045
DOI: 10.1016/j.jplph.2009.02.005

Abstract

The SMALL ACIDIC PROTEIN 2 (SMAP2) gene is a paralogue of the SMAP1 gene that mediates the response to the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) in the root of Arabidopsis thaliana. Their encoded proteins, SMAP1 and SMAP2, are similar in calculated molecular weight and isoelectric point, and in having a highly conserved phenylalanine and aspartic acid-rich domain. RNA expression analysis showed that SMAP1 mRNA is present throughout the plant body while SMAP2 mRNA is restricted to siliques and anthers. Over-expression of the SMAP2 gene, as well as SMAP1, by 35S cauliflower mosaic virus promoter restored sensitivity to 2,4-D in the 2,4-D-resistant mutant, aar1, which is defective in SMAP1 function. The results suggest that SMAP2 has an ability to mediate the 2,4-D response and is expressed only in restricted tissues.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

2,4-Dichlorophenoxyacetic Acid / pharmacology*
Amino Acid Sequence
Arabidopsis / drug effects*
Arabidopsis / genetics*
Arabidopsis Proteins / chemistry
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Chromosomes, Plant / genetics
Gene Expression Regulation, Plant / drug effects
Genes, Plant*
Indoleacetic Acids / pharmacology*
Molecular Sequence Data
Sequence Homology, Amino Acid

Substances

Arabidopsis Proteins
Indoleacetic Acids
SMAP1 protein, Arabidopsis
2,4-Dichlorophenoxyacetic Acid