Functional analysis of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin macrolide antibiotics

Chembiochem. 2009 May 25;10(8):1297-301. doi: 10.1002/cbic.200900088.

Abstract

Mg motors: We characterized the in vitro function of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin antibiotics. Each enzyme was confirmed to be an S-adenosyl-L-methionine (SAM)-dependent deoxysugar methyltransferase. Their optimal activities require the presence of Mg(2+). With the reconstituted in vitro assays, the order of mycinamicin VI-->III-->IV in the post-PKS (polyketide synthase) tailoring pathway of mycinamicin was unambiguously determined.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents / biosynthesis*
  • Anti-Bacterial Agents / chemistry
  • Kinetics
  • Macrolides / chemistry*
  • Magnesium / metabolism
  • Methyltransferases / metabolism
  • Molecular Sequence Data
  • Multigene Family
  • Phylogeny
  • Polyketide Synthases / metabolism
  • Protein O-Methyltransferase / chemistry*
  • Protein O-Methyltransferase / classification
  • Protein O-Methyltransferase / metabolism
  • Sequence Homology, Amino Acid

Substances

  • Anti-Bacterial Agents
  • Macrolides
  • mycinamicins
  • Polyketide Synthases
  • Methyltransferases
  • Protein O-Methyltransferase
  • S-adenosyl-L-methionine (R,S)-reticuline 7-O-methyltransferase
  • Magnesium