Abstract
Mg motors: We characterized the in vitro function of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin antibiotics. Each enzyme was confirmed to be an S-adenosyl-L-methionine (SAM)-dependent deoxysugar methyltransferase. Their optimal activities require the presence of Mg(2+). With the reconstituted in vitro assays, the order of mycinamicin VI-->III-->IV in the post-PKS (polyketide synthase) tailoring pathway of mycinamicin was unambiguously determined.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / biosynthesis*
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Anti-Bacterial Agents / chemistry
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Kinetics
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Macrolides / chemistry*
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Magnesium / metabolism
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Methyltransferases / metabolism
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Molecular Sequence Data
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Multigene Family
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Phylogeny
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Polyketide Synthases / metabolism
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Protein O-Methyltransferase / chemistry*
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Protein O-Methyltransferase / classification
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Protein O-Methyltransferase / metabolism
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Sequence Homology, Amino Acid
Substances
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Anti-Bacterial Agents
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Macrolides
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mycinamicins
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Polyketide Synthases
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Methyltransferases
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Protein O-Methyltransferase
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S-adenosyl-L-methionine (R,S)-reticuline 7-O-methyltransferase
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Magnesium