Abstract
Defective interfering (DI) particles of vesicular stomatitis virus which contain covalently linked complementary [+]message and [-]anti-message RNA as a single-stranded ribonucleoprotein complex within the particle, are extremely efficient inducers of interferon. A single particle can induce a quantum yield of interferon. A single molecule of double-stranded RNA presumed to form, at least in part, on entry into the cell is thought to induce interferon synthesis. Conventional [-]RNA DI particles with the same polypeptide composition as [+/-]RNA DI particles fail to induce interferon.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Viral
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Antigen-Antibody Reactions
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Cell Line
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Cell Survival
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Cycloheximide / pharmacology
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Dactinomycin / pharmacology
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Defective Viruses / metabolism*
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Defective Viruses / radiation effects
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Hot Temperature
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Interferons / biosynthesis*
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Nucleic Acid Conformation
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RNA, Viral / metabolism*
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Templates, Genetic
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Ultraviolet Rays
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Vesicular stomatitis Indiana virus / immunology
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Vesicular stomatitis Indiana virus / metabolism*
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Vesicular stomatitis Indiana virus / radiation effects
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Viral Interference
Substances
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Antibodies, Viral
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RNA, Viral
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Dactinomycin
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Interferons
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Cycloheximide