Cloning and functional analysis of cis-prenyltransferase from Thermobifida fusca

Takanori Ambo; Motoyoshi Noike; Hirofumi Kurokawa; Tanetoshi Koyama

doi:10.1016/j.jbiosc.2009.02.006

Cloning and functional analysis of cis-prenyltransferase from Thermobifida fusca

J Biosci Bioeng. 2009 Jun;107(6):620-2. doi: 10.1016/j.jbiosc.2009.02.006.

Authors

Takanori Ambo¹, Motoyoshi Noike, Hirofumi Kurokawa, Tanetoshi Koyama

Affiliation

¹ Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan.

PMID: 19447338
DOI: 10.1016/j.jbiosc.2009.02.006

Abstract

cis-Prenyltransferase catalyzes the synthesis of Z,E-mixed prenyl diphosphates by a condensation of isopentenyl diphosphate to an allylic diphosphate. A novel gene encoding a cis-prenyltransferase is cloned from Thermobifida fusca. It showed a unique substrate specificity accepting dimethylallyl diphosphate as a shortest allylic substrate, and synthesizes polyprenyl products up to C(70).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actinomycetales / enzymology*
Bacterial Proteins / isolation & purification
Bacterial Proteins / metabolism*
Cloning, Molecular
Substrate Specificity
Transferases / isolation & purification
Transferases / metabolism*

Substances

Bacterial Proteins
Transferases
cis-prenyl transferase