Since the discovery of the endocannabinoids anandamide and 2-arachidonoylglycerol (2-AG) in the early 1990s, the endocannabinoid system has been implicated in a wide array of physiological processes, such as control of food intake and energy balance, fertility and obesity. As the importance of this system becomes apparent, there is a tremendous need for robust, sensitive and efficient analytical methodology for the examination of the endocannabinoids, their congeners and putative metabolites. This review will summarize quantitative analytical methodology as reported in the literature from 1992 to present for the analysis of endocannabinoids and related compounds.