A radiorespirometric method for measuring mineralization of [14C]-compounds in a 96-well microplate format

Anders R Johnsen; Trine K Hybholt; Ole S Jacobsen; Jens Aamand

doi:10.1016/j.mimet.2009.07.018

A radiorespirometric method for measuring mineralization of [14C]-compounds in a 96-well microplate format

J Microbiol Methods. 2009 Oct;79(1):114-6. doi: 10.1016/j.mimet.2009.07.018. Epub 2009 Jul 25.

Authors

Anders R Johnsen¹, Trine K Hybholt, Ole S Jacobsen, Jens Aamand

Affiliation

¹ Geological Survey of Denmark and Greenland (GEUS), Department of Geochemistry, Øster Voldgade 10, DK-1350 Copenhagen K, Denmark. arj@geus.dk

PMID: 19635506
DOI: 10.1016/j.mimet.2009.07.018

Abstract

A radiorespirometric method was developed for quantifying mineralization of [14C]-labeled substrates in deep-well microplates. Ninety-six 14CO2-traps were placed on an adhesive microplate sealing tape which made it possible to trap 14CO2 simultaneously from all wells. Trapped radioactivity was quantified by digital autoradiography.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbon Dioxide / metabolism*
Carbon Radioisotopes / metabolism*
Environmental Microbiology*
Environmental Monitoring
Staining and Labeling / methods*

Substances

Carbon Radioisotopes
Carbon Dioxide