Non-susceptibility genes to Bombyx densovirus type 1, Nid-1 and nsd-1, affect distinct steps of the viral infection pathway

Kurako Kidokoro; Katsuhiko Ito; Dorington O Ogoyi; Hiroaki Abe; Kazuei Mita; Keiko Kadono-Okuda

doi:10.1016/j.jip.2009.10.006

Non-susceptibility genes to Bombyx densovirus type 1, Nid-1 and nsd-1, affect distinct steps of the viral infection pathway

J Invertebr Pathol. 2010 Jan;103(1):79-81. doi: 10.1016/j.jip.2009.10.006. Epub 2009 Oct 27.

Authors

Kurako Kidokoro¹, Katsuhiko Ito, Dorington O Ogoyi, Hiroaki Abe, Kazuei Mita, Keiko Kadono-Okuda

Affiliation

¹ Division of Insect Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.

PMID: 19836396
DOI: 10.1016/j.jip.2009.10.006

Abstract

In the silkworm Bombyx mori, densovirus type 1 (BmDNV-1) replicates only in the midgut and causes fatal disease. Resistance to BmDNV-1 is determined by two genes, nsd-1 and Nid-1, respectively. Neither of them has been identified yet. We investigated the viral transcript by RT-PCR in inoculated silkworms carrying different sets of nsd-1 and Nid-1 genotype. BmDNV-1 transcript was not detected in nsd-1-carrying strains irrespective of existence of Nid-1 but clearly detected in strains carrying Nid-1 without nsd-1. The result suggests that nsd-1 blocks early step of infection. On the other hand, Nid-1 does not block cell and nucleus entry and viral transcription in nuclei but blocks later step in the viral infection cycle.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bombyx / genetics*
Bombyx / virology*
Densovirus / pathogenicity*
Digestive System / virology
Genes, Insect*
Genetic Predisposition to Disease / genetics*
Genotype
Insect Viruses / pathogenicity