From selection to caged aptamers: identification of light-dependent ssDNA aptamers targeting cytohesin

Günter Mayer; Andrea Lohberger; Sabine Butzen; Monika Pofahl; Michael Blind; Alexander Heckel

doi:10.1016/j.bmcl.2009.10.032

From selection to caged aptamers: identification of light-dependent ssDNA aptamers targeting cytohesin

Bioorg Med Chem Lett. 2009 Dec 1;19(23):6561-4. doi: 10.1016/j.bmcl.2009.10.032. Epub 2009 Oct 14.

Authors

Günter Mayer¹, Andrea Lohberger, Sabine Butzen, Monika Pofahl, Michael Blind, Alexander Heckel

Affiliation

¹ Strathclyde Institute for Pharmacy and Biological Sciences, University of Strathclyde, Glasgow G4 0NR, Scotland, United Kingdom. gunter.mayer@strath.ac.uk

PMID: 19854646
DOI: 10.1016/j.bmcl.2009.10.032

Abstract

Caged aptamers represent valuable tools for the spatiotemporal control of protein function by light. Here we describe a general route starting with the de novo selection process targeting cytohesin-1 and aiming at the synthesis of caged aptamers without the prior knowledge of detailed structural determinants of aptamer-target binding.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemical synthesis
Aptamers, Nucleotide / chemistry
Aptamers, Nucleotide / pharmacology*
Base Sequence
Binding Sites
DNA, Single-Stranded / chemical synthesis
DNA, Single-Stranded / chemistry
DNA, Single-Stranded / pharmacology*
Guanine Nucleotide Exchange Factors / antagonists & inhibitors*
Guanine Nucleotide Exchange Factors / chemistry
Light*
Molecular Sequence Data
Structure-Activity Relationship

Substances

Aptamers, Nucleotide
DNA, Single-Stranded
Guanine Nucleotide Exchange Factors
cytohesin-1