Aptamers as a model for functional evaluation of LNA and 2'-amino LNA

Frank J Hernandez; Neerja Kalra; Jesper Wengel; Birte Vester

doi:10.1016/j.bmcl.2009.10.039

Aptamers as a model for functional evaluation of LNA and 2'-amino LNA

Bioorg Med Chem Lett. 2009 Dec 1;19(23):6585-7. doi: 10.1016/j.bmcl.2009.10.039. Epub 2009 Oct 13.

Authors

Frank J Hernandez¹, Neerja Kalra, Jesper Wengel, Birte Vester

Affiliation

¹ Biochemistry and Molecular Biology Department, Nucleic Acid Center, University of Southern Denmark, Campusvej 55, Odense M 5230, Denmark.

PMID: 19864131
DOI: 10.1016/j.bmcl.2009.10.039

Abstract

The affinity change upon incorporation of LNA and 2'-amino-LNA monomers into an avidin binding DNA aptamer is described. The kinetic profile of selected modified-aptamer was obtained by surface plasmon resonance experiments and compared with the profile of the parent unmodified DNA aptamer. We report significant improvement of avidin binding affinity by the incorporation of single LNA modifications into the aptamer, and successful incorporation of 2'-amino LNA as a novel monomer in aptamers with potential function as carrier unit for additional molecular entities.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Avidin / chemistry
Binding Sites
Kinetics
Models, Chemical*
Oligonucleotides / chemistry*
Surface Plasmon Resonance
Time Factors

Substances

Aptamers, Nucleotide
Oligonucleotides
locked nucleic acid
Avidin