Interaction of afobazole with sigma1-receptors

S B Seredenin; T A Antipova; M V Voronin; S Yu Kurchashova; A N Kuimov

doi:10.1007/s10517-009-0624-x

Interaction of afobazole with sigma1-receptors

Bull Exp Biol Med. 2009 Jul;148(1):42-4. doi: 10.1007/s10517-009-0624-x.

Authors

S B Seredenin¹, T A Antipova, M V Voronin, S Yu Kurchashova, A N Kuimov

Affiliation

¹ V. V. Zakusov Institute of Pharmacology, Russian Academy of Medical Sciences, M. V. Lomonosov Moscow State University, Russia.

PMID: 19902093
DOI: 10.1007/s10517-009-0624-x

Abstract

In vitro radioligand assay revealed interaction of afobazole with sigma(1)-receptors (Ki=5.9x10(-6) M). Translocation of sigma(1)-receptors from the endoplasmic reticulum to the outer membrane was demonstrated by confocal microscopy. Experiments were performed on the model of HT-22 immortalized hippocampal cells after incubation with afobazole in a concentration of 10(-8) M.

MeSH terms

Animals
Anti-Anxiety Agents / pharmacology*
Benzimidazoles / pharmacology*
Cell Line, Transformed
Fluorescent Antibody Technique
Hippocampus / cytology
Hippocampus / drug effects
Humans
Jurkat Cells
Mice
Morpholines / pharmacology*
Neuroprotective Agents / pharmacology*
Radioligand Assay
Receptors, sigma / drug effects*
Sigma-1 Receptor

Substances

2-((2-morpholino)ethylthio)-5-ethoxybenzimidazole
Anti-Anxiety Agents
Benzimidazoles
Morpholines
Neuroprotective Agents
Receptors, sigma