Measurement of oxidized lipoprotein (a) in patients with acute coronary syndromes and stable coronary artery disease by 2 ELISAs: using different capture antibody against oxidized lipoprotein (a) or oxidized LDL

Clin Biochem. 2010 Apr;43(6):571-5. doi: 10.1016/j.clinbiochem.2009.12.017. Epub 2010 Jan 7.

Abstract

Objective: To evaluate clinical value of oxidized lipoprotein(a) [ox-Lp(a)] levels.

Design and methods: Ox-Lp(a) were measured by 2 ELISAs using antibodies against ox-Lp(a) [ox-Lp(a)1] or oxidized low-density lipoprotein [ox-Lp(a)2], and studied in 161 acute coronary syndromes (ACS) patients, 114 stable coronary artery disease (CAD) and 100 control subjects.

Results: Ox-Lp(a)1 was found related with ox-Lp(a)2 (r=0.864, P=0.000). Controlling for plasma lipids, Lp(a) and clinical characteristics, odds ratios of ox-Lp(a)1 on ACS and stable CAD were 5.06 (95% confidence interval 1.82-14.04) and 2.20 (0.78-6.22); those of ox-Lp(a)2 were 3.37 (1.07-10.63) and 1.35 (0.41-4.48), respectively. Receiver-operating characteristic curve analysis confirmed that performances of ox-Lp(a)1 were significantly superior to those for ox-Lp(a)2 in ACS (area: 0.803 vs. 0.723, P<0.001) and stable CAD (area: 0.670 vs. 0.607, P<0.01).

Conclusion: Ox-Lp(a) levels using antibodies against ox-Lp(a) may represent a better risk marker than those using antibodies against oxidized low-density lipoprotein for ACS and stable CAD.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Coronary Syndrome / blood*
  • Acute Coronary Syndrome / diagnosis
  • Acute Coronary Syndrome / metabolism
  • Aged
  • Antibodies / pharmacology*
  • Case-Control Studies
  • Coronary Artery Disease / blood*
  • Coronary Artery Disease / diagnosis
  • Coronary Artery Disease / metabolism
  • Disease Progression
  • Enzyme-Linked Immunosorbent Assay / methods
  • Female
  • Humans
  • Lipoprotein(a) / analysis*
  • Lipoprotein(a) / blood
  • Lipoprotein(a) / immunology*
  • Lipoprotein(a) / metabolism
  • Lipoproteins, LDL / analysis
  • Lipoproteins, LDL / blood
  • Lipoproteins, LDL / immunology*
  • Male
  • Middle Aged
  • Oxidation-Reduction
  • Predictive Value of Tests
  • ROC Curve
  • Severity of Illness Index

Substances

  • Antibodies
  • Lipoprotein(a)
  • Lipoproteins, LDL
  • oxidized low density lipoprotein