Improved ethanol precipitation of DNA

Electrophoresis. 2010 Apr;31(8):1350-2. doi: 10.1002/elps.200900721.

Abstract

In this Short Communication, a shorter version of the standard DNA ethanol precipitation and purification protocol is described. It uses a mixture of 70% ethanol, 75 mM ammonium acetate and different concentrations of different carriers to perform DNA precipitation and washing in only one step.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates / chemistry
  • Acrylamide / chemistry
  • Chemical Precipitation
  • DNA / chemistry
  • DNA / isolation & purification*
  • DNA, Mitochondrial
  • Electrophoresis, Agar Gel / methods*
  • Ethanol / chemistry*
  • Glycogen / chemistry
  • Humans
  • Polymerase Chain Reaction
  • Temperature

Substances

  • Acetates
  • DNA, Mitochondrial
  • Acrylamide
  • Ethanol
  • Glycogen
  • DNA
  • ammonium acetate