Abstract
In this Short Communication, a shorter version of the standard DNA ethanol precipitation and purification protocol is described. It uses a mixture of 70% ethanol, 75 mM ammonium acetate and different concentrations of different carriers to perform DNA precipitation and washing in only one step.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetates / chemistry
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Acrylamide / chemistry
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Chemical Precipitation
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DNA / chemistry
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DNA / isolation & purification*
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DNA, Mitochondrial
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Electrophoresis, Agar Gel / methods*
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Ethanol / chemistry*
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Glycogen / chemistry
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Humans
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Polymerase Chain Reaction
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Temperature
Substances
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Acetates
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DNA, Mitochondrial
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Acrylamide
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Ethanol
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Glycogen
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DNA
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ammonium acetate