Multicenter evaluation of a new shortened peptide nucleic acid fluorescence in situ hybridization procedure for species identification of select Gram-negative bacilli from blood cultures

Margie Morgan; Elizabeth Marlowe; Phyllis Della-Latta; Hossein Salimnia; Susan Novak-Weekley; Fann Wu; Benjamin S Crystal

doi:10.1128/JCM.00166-10

Multicenter evaluation of a new shortened peptide nucleic acid fluorescence in situ hybridization procedure for species identification of select Gram-negative bacilli from blood cultures

J Clin Microbiol. 2010 Jun;48(6):2268-70. doi: 10.1128/JCM.00166-10. Epub 2010 Mar 31.

Authors

Margie Morgan¹, Elizabeth Marlowe, Phyllis Della-Latta, Hossein Salimnia, Susan Novak-Weekley, Fann Wu, Benjamin S Crystal

Affiliation

¹ Cedars Sinai Medical Center, Los Angeles, California, USA.

Abstract

A shortened protocol for two peptide nucleic acid fluorescence in situ hybridization (PNA FISH) assays for the detection of Gram-negative bacilli from positive blood cultures was evaluated in a multicenter trial. There was 100% concordance between the two protocols for each assay (368 of 368 and 370 of 370 results) and 99.7% (367 of 368 and 369 of 370 results) agreement with routine laboratory techniques.

Publication types

Evaluation Study
Multicenter Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques / methods*
Blood / microbiology*
Gram-Negative Bacteria / classification
Gram-Negative Bacteria / isolation & purification*
Gram-Negative Bacterial Infections / diagnosis*
Gram-Negative Bacterial Infections / microbiology
Humans
In Situ Hybridization, Fluorescence / methods*
Peptide Nucleic Acids*
Sensitivity and Specificity

Substances

Peptide Nucleic Acids