The organization of GABAergic elements in the histaminergic tuberomammillary nucleus has been examined by using antibodies against gamma-aminobutyric acid (GABA) and light and electron microscopy. Most neuronal perikarya of the ventral subgroup of the tuberomammillary nucleus were GABA immunoreactive (GABA-i). The morphology of the GABA-i perikarya was similar to the morphology of histaminergic perikarya described by Hayashi et al. ('84: J. Comp. Neurol. 229: 223-241) and Wouterlood et al. ('86: J. Comp. Neurol. 252:227-243). The GABA-i perikarya were contacted by relatively few terminals. The mean bouton covering ratio of GABA-i perikarya was 6.1%, whereas the mean bouton covering ratio for GABA-i dendrites in the tuberomammillary nucleus was 31%. Some of the presynaptic terminals were GABA-i. In addition, GABA-i perikarya and dendrites formed close contacts that never presented synaptic specializations. These results suggest that neurons of the histaminergic tuberomammillary nucleus contain the neurotransmitter GABA. Furthermore, GABA may act as a modulator of cellular processes within the tuberomammillary nucleus.