Abstract
The process of chromosome duplication faces many obstacles. One way to circumvent blocks is to hop over them by placing a new clamp on a downstream primer. This resembles lagging strand synthesis, where the tight grip of polymerase to the clamp and DNA must be overcome upon completing each Okazaki fragment so it can transfer to new primed sites. This review focuses on recent single-molecule studies showing that Escherichia coli Pol III can hop from one clamp to another without leaving the replication fork. This capability provides a means to circumvent obstacles like transcription or DNA lesions without fork collapse.
Copyright 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
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Review
MeSH terms
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Chromosomes, Bacterial / genetics
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Chromosomes, Bacterial / metabolism
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DNA / genetics
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DNA / metabolism
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DNA Polymerase III / chemistry
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DNA Polymerase III / metabolism
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DNA Replication / physiology*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Bacterial / metabolism
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DnaB Helicases / chemistry
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DnaB Helicases / metabolism
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli Proteins / chemistry
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Escherichia coli Proteins / metabolism
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Models, Biological
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Models, Molecular
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Nucleic Acid Conformation
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Protein Conformation
Substances
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DNA, Bacterial
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Escherichia coli Proteins
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Okazaki fragments
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DNA
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DNA Polymerase III
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dnaB protein, E coli
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DnaB Helicases