Computer simulation and analysis of the reaction pathway of triosephosphate isomerase

Biochemistry. 1991 Jun 18;30(24):5826-32. doi: 10.1021/bi00238a003.

Abstract

A theoretical approach designed for chemical reactions in the condensed phase is used to determine the energy along the reaction path of the enzyme triosephosphate isomerase. The calculations address the role of the enzyme in lowering the barrier to reaction and provide a decomposition into specific residue contributions. The results suggest that, although Lys-12 is most important, many other residues within 16 A of the substrate contribute and that histidine-95 as the imidazole/imidazolate pair could act as an acid/base catalyst.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Computer Simulation
  • Dihydroxyacetone Phosphate / chemistry
  • Dihydroxyacetone Phosphate / metabolism
  • Energy Transfer
  • Glyceraldehyde 3-Phosphate / chemistry
  • Glyceraldehyde 3-Phosphate / metabolism
  • Models, Molecular
  • Models, Theoretical
  • Molecular Conformation
  • Molecular Structure
  • Protein Conformation
  • Saccharomyces cerevisiae / enzymology
  • Triose-Phosphate Isomerase / chemistry
  • Triose-Phosphate Isomerase / metabolism*
  • X-Ray Diffraction

Substances

  • Glyceraldehyde 3-Phosphate
  • Dihydroxyacetone Phosphate
  • Triose-Phosphate Isomerase