Abstract
Two bacterial beta1-4-galactosyltransferases, NmLgtB and Hp1-4GalT, exhibit promiscuous and complementary acceptor substrate specificity. They have been used in an efficient one-pot multienzyme system to synthesize LacNAc, lactose, and their derivatives including those containing negatively charged 6-O-sulfated GlcNAc and C2-substituted GlcNAc or Glc, from monosaccharide derivatives and inexpensive Glc-1-P.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Carbohydrate Conformation
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Carbohydrate Sequence
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Cloning, Molecular
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Escherichia coli / genetics
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Galactosides / chemical synthesis*
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Galactosides / chemistry
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Helicobacter pylori / enzymology*
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N-Acetyllactosamine Synthase / chemistry*
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N-Acetyllactosamine Synthase / genetics
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Neisseria meningitidis / enzymology*
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Pasteurella multocida / enzymology*
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Recombinant Fusion Proteins / chemistry*
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Recombinant Fusion Proteins / genetics
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Substrate Specificity
Substances
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Galactosides
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Recombinant Fusion Proteins
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N-Acetyllactosamine Synthase