Molecular identification of Na(+)-H(+) exchanger isoforms (NHE2) in the gills of the euryhaline teleost Fundulus heteroclitus

J Fish Biol. 2010 Feb;76(2):415-26. doi: 10.1111/j.1095-8649.2009.02534.x.

Abstract

In the current study, reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR were used to clone full-length putative Na(+)-H(+) exchanger isoforms (NHE2a) cDNA from the gills of Fundulus heteroclitus. The 2480 bp cDNA includes a coding region for a protein that shows a 57% amino acid homology to rabbit NHE2. These sequences allowed data mining of available fish genome data, which revealed at least three NHE2 subtypes in some teleost species.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Fundulidae / classification
  • Fundulidae / metabolism*
  • Gills / metabolism*
  • In Situ Hybridization
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Alignment
  • Sodium-Hydrogen Exchangers / chemistry
  • Sodium-Hydrogen Exchangers / genetics*
  • Sodium-Hydrogen Exchangers / metabolism*

Substances

  • Sodium-Hydrogen Exchangers

Associated data

  • GENBANK/AAV68496
  • GENBANK/EU886295