Crystallization of a chymotrypsin inhibitor from Erythrina caffra seeds

H S Shieh; N K Leimgruber; M O Palmier; T C Wun; R M Leimgruber; S S Abdel-Meguid

doi:10.1016/0022-2836(90)90300-B

Crystallization of a chymotrypsin inhibitor from Erythrina caffra seeds

J Mol Biol. 1990 Mar 5;212(1):15-6. doi: 10.1016/0022-2836(90)90300-B.

Authors

H S Shieh¹, N K Leimgruber, M O Palmier, T C Wun, R M Leimgruber, S S Abdel-Meguid

Affiliation

¹ Corporate Research and Development, Monsanto, Chesterfield Village, MO 63198.

PMID: 2108250
DOI: 10.1016/0022-2836(90)90300-B

Abstract

Crystals of a chymotrypsin inhibitor from Erythrina caffra seeds have been grown out of lithium sulfate, by the hanging drop method of vapor diffusion. The crystals belong to the rhombohedral space group R32, with a = 67.2 A and alpha = 99.4 degrees, and diffract to 3 A resolution.

MeSH terms

Chromatography, Affinity
Chymotrypsin / antagonists & inhibitors*
Crystallization
Erythrina / analysis*
Molecular Weight
Plant Proteins / isolation & purification*
Plants, Medicinal / analysis*
Seeds / analysis
Tissue Plasminogen Activator / antagonists & inhibitors

Substances

Plant Proteins
Chymotrypsin
Tissue Plasminogen Activator