Abstract
Mycobacterium indicus pranii (MIP), also known as Mw, is a saprophytic, non-pathogenic strain of Mycobacterium and is commercially available as a heat-killed vaccine for leprosy and recently tuberculosis (TB) as part of MDT. In this study we provide evidence that cell-free supernatant collected from original MIP suspension induces rapid and enhanced apoptosis in mouse peritoneal macrophages in vitro. It is demonstrated that the MIP cell-free supernatant induced apoptosis is mitochondria-mediated and caspase independent and involves mitochondrial translocation of Bax and subsequent release of AIF and cytochrome c from the mitochondria. Experiments with pharmacological inhibitors suggest a possible role of PKC in mitochondria-mediated apoptosis of macrophages.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus / drug effects
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Animals
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Apoptosis / drug effects*
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Apoptosis Inducing Factor / metabolism
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Bacterial Vaccines / adverse effects
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Caspase 3 / metabolism
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Cell Nucleus / drug effects
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Cell Nucleus / metabolism
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Culture Media, Conditioned / metabolism
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Culture Media, Conditioned / pharmacology
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Cytochromes c / metabolism
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Down-Regulation / drug effects
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Female
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Injections
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Lipopolysaccharides / pharmacology
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Macrophages, Peritoneal / cytology*
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Macrophages, Peritoneal / drug effects*
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Macrophages, Peritoneal / metabolism
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Male
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Mice
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Mice, Inbred BALB C
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Mitochondria / drug effects
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Mitochondria / metabolism
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Mycobacterium / cytology*
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Mycobacterium / immunology
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Poly (ADP-Ribose) Polymerase-1
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Poly(ADP-ribose) Polymerases / metabolism
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Protein Kinase C-delta / antagonists & inhibitors
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Protein Kinase C-delta / metabolism
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Protein Kinase Inhibitors / pharmacology
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Proteolysis / drug effects
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Proteomics
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bcl-2-Associated X Protein / metabolism
Substances
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Apoptosis Inducing Factor
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Bacterial Vaccines
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Culture Media, Conditioned
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Lipopolysaccharides
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AIFM1 protein, mouse
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Protein Kinase Inhibitors
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bcl-2-Associated X Protein
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Cytochromes c
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Parp1 protein, mouse
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Poly (ADP-Ribose) Polymerase-1
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Poly(ADP-ribose) Polymerases
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Protein Kinase C-delta
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Caspase 3