Abstract
Zuo-Jin-Wan (ZJW) has been used to treat hepatocellular carcinoma in Asia. This study was to determine whether ZJW and its components blocked activator protein 1 (AP-1) and nuclear factor-κB (NF-κB) activities as well as tumor promotion in hepatoblastoma HepG2 cells. ZJW and its components, Coptis chinensis and Evodia rutaecarpa, inhibited AP-1 and NF-κB activities, and suppressed anchorage-independent growth of HepG2 cells. The major alkaloidal ingredients, berberine and evodiamine, inhibited AP-1 activities and/or NF-κB activation, and further suppressed hepatocellular transformation. In conclusion, ZJW and its constituents, berberine and evodiamine, suppressed tumor promotion primarily through AP-1 and/or NF-κB pathways in HepG2 cells.
Copyright © 2011 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / pharmacology*
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Antineoplastic Agents, Phytogenic / therapeutic use
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Berberine / isolation & purification
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Berberine / pharmacology
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Carcinoma, Hepatocellular / drug therapy
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Cell Transformation, Neoplastic / chemically induced
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Cell Transformation, Neoplastic / drug effects*
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Coptis / chemistry
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Drugs, Chinese Herbal / chemistry
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Drugs, Chinese Herbal / pharmacology*
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Drugs, Chinese Herbal / therapeutic use
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Evodia / chemistry
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Hep G2 Cells
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Humans
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Liver Neoplasms / drug therapy
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NF-kappa B / drug effects*
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Plant Extracts / pharmacology
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Quinazolines / isolation & purification
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Quinazolines / pharmacology
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Tetradecanoylphorbol Acetate
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Transcription Factor AP-1 / drug effects*
Substances
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Antineoplastic Agents, Phytogenic
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Drugs, Chinese Herbal
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NF-kappa B
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Plant Extracts
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Quinazolines
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Transcription Factor AP-1
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Zuo-Jin-Wan
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Berberine
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evodiamine
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Tetradecanoylphorbol Acetate