The frequency of mouse spleen dendritic cells which present alloantigens or ovalbumin to primed T lymphocytes is equal

Cell Immunol. 1990 Apr 1;126(2):406-13. doi: 10.1016/0008-8749(90)90331-k.

Abstract

We have used limiting dilution analysis to compare the frequency of dendritic cells (DC) which present endogenous alloantigens with that which present an exogenous protein antigen to T lymphocytes. Spleen DC present alloantigens or ovalbumin to primed T lymphocytes with equal frequency, showing that DC are equipotent for presenting endogenous and exogenous antigens. Also, antigen-presenting cell (APC) frequencies among DC were compared with other APC populations. DC were enriched about 1000-fold for APC compared to unfractionated spleen cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen-Presenting Cells / immunology
  • Dendritic Cells / immunology*
  • Histocompatibility Antigens Class II / analysis
  • Isoantigens / immunology*
  • Lymphocyte Culture Test, Mixed
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Ovalbumin / immunology*
  • Spleen / immunology
  • T-Lymphocytes / immunology*

Substances

  • Histocompatibility Antigens Class II
  • Isoantigens
  • Ovalbumin