Effect of all-trans retinoic acid on the barrier function in human retinal pigment epithelial cells

Junbo Rong; Shuangzhen Liu

doi:10.1016/j.bbrc.2011.03.080

Effect of all-trans retinoic acid on the barrier function in human retinal pigment epithelial cells

Biochem Biophys Res Commun. 2011 Apr 15;407(3):605-9. doi: 10.1016/j.bbrc.2011.03.080. Epub 2011 Mar 21.

Authors

Junbo Rong¹, Shuangzhen Liu

Affiliation

¹ Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, People's Republic of China.

PMID: 21426899
DOI: 10.1016/j.bbrc.2011.03.080

Abstract

To investigate the effects of all-trans retinoic acid (atRA) on the barrier function in human retinal pigment epithelial cells, ARPE-19 cells were cultured on the filters as monolayer with atRA being added in the apical side. The change of epithelial permeability was observed from the measurement of transepithelial electrical resistance (TER), permeability assay, and Western Blot analysis. We discovered that atRA promoted the epithelial barrier function in vitro, and its bioavailability regulates the epithelial barrier, which is accompanied by altering expression of tight junctions (TJ)-associated proteins. Our study indicates that atRA provides barrier-positive elements to the RPE cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Cell Membrane Permeability / drug effects
Humans
Retinal Pigment Epithelium / drug effects*
Retinal Pigment Epithelium / metabolism*
Tight Junctions / drug effects
Tight Junctions / metabolism
Tretinoin / pharmacology*

Substances

Tretinoin