Abstract
Serum-free mouse embryo cells cultured in medium supplemented with insulin, transferrin, high-density lipoprotein, and fibronectin are dependent on epidermal growth factor for survival. Cycloheximide or actinomycin D prevented cell death caused by growth factor deprivation, suggesting that cell death required the synthesis of RNA and protein, a phenomenon similar to that reported for neuronal cell death in the absence of nerve growth factor. Orthovanadate, an inhibitor of phosphotyrosine phosphatases, and 12-O-tetradecanoylphorbol-13-acetate, an activator of protein kinase C, also prevented serum-free mouse embryo cell death in the absence of epidermal growth factor.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adenylyl Cyclases / metabolism
-
Animals
-
Cell Survival / drug effects
-
Cycloheximide / pharmacology*
-
Embryo, Mammalian / cytology*
-
Embryo, Mammalian / drug effects
-
Embryo, Mammalian / ultrastructure
-
Epidermal Growth Factor / drug effects
-
Epidermal Growth Factor / pharmacology*
-
ErbB Receptors / metabolism
-
Mice
-
Nerve Growth Factors / pharmacology
-
Neurons / drug effects
-
Neurons / ultrastructure
-
Phorbol Esters / pharmacology
-
Phosphoprotein Phosphatases / metabolism
-
Phosphorylation
-
Potassium / pharmacology
-
Protein Kinase C / metabolism
-
Protein Tyrosine Phosphatases
-
Tetradecanoylphorbol Acetate / pharmacology*
-
Tretinoin / pharmacology
-
Vanadates / pharmacology*
Substances
-
Nerve Growth Factors
-
Phorbol Esters
-
Vanadates
-
Tretinoin
-
Epidermal Growth Factor
-
Cycloheximide
-
ErbB Receptors
-
Protein Kinase C
-
Phosphoprotein Phosphatases
-
Protein Tyrosine Phosphatases
-
Adenylyl Cyclases
-
Tetradecanoylphorbol Acetate
-
Potassium