Evaluation of sperm proteins in infertile men: a proteomic approach

Fertil Steril. 2011 Jun 30;95(8):2745-8. doi: 10.1016/j.fertnstert.2011.03.112. Epub 2011 May 4.

Abstract

In this study, the sperm protein profile was compared between fertile and infertile men using 2-dimensional gel electrophoresis, liquid chromatography mass spectrometer analysis, and matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry. Four unique proteins, semenogelin II precursor, prolactin-induced protein, clusterin isoform 1, and prostate-specific antigen isoform 1 preproprotein, were predominantly present in the semen of healthy men; however, semenogelin II precursor and clusterin isoform 1 were not seen in the semen of infertile men, suggesting unique differences in the spermatozoa protein profiles of fertile and infertile men.

MeSH terms

  • Amino Acid Sequence
  • Biomarkers / analysis
  • Carrier Proteins / analysis
  • Case-Control Studies
  • Chromatography, Reverse-Phase
  • Clusterin / analysis
  • Electrophoresis, Gel, Two-Dimensional
  • Fertility*
  • Glycoproteins / analysis
  • Humans
  • Infertility, Male / metabolism*
  • Infertility, Male / physiopathology
  • Male
  • Membrane Transport Proteins
  • Molecular Weight
  • Ohio
  • Pilot Projects
  • Prostate-Specific Antigen / analysis
  • Proteomics* / methods
  • Seminal Plasma Proteins / analysis*
  • Seminal Plasma Proteins / chemistry
  • Seminal Vesicle Secretory Proteins / analysis
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Spermatozoa / chemistry*

Substances

  • Biomarkers
  • CLU protein, human
  • Carrier Proteins
  • Clusterin
  • Glycoproteins
  • Membrane Transport Proteins
  • PIP protein, human
  • Seminal Plasma Proteins
  • Seminal Vesicle Secretory Proteins
  • seminal vesicle-specific antigen
  • Prostate-Specific Antigen