Development of polymorphic microsatellite markers for the livebearing fish Poecilia parae

Alexander Nater; Michael Krützen; Anna K Lindholm

doi:10.1111/j.1755-0998.2008.02090.x

Development of polymorphic microsatellite markers for the livebearing fish Poecilia parae

Mol Ecol Resour. 2008 Jul;8(4):857-60. doi: 10.1111/j.1755-0998.2008.02090.x.

Authors

Alexander Nater¹, Michael Krützen, Anna K Lindholm

Affiliation

¹ Anthropological Institute and Museum, University of Zurich, Winterthurerstrasse 190, 8057 Zürich, Switzerland, Zoological Institute, University of Zurich, Winterthurerstrasse 190, 8057 Zürich, Switzerland.

PMID: 21585912
DOI: 10.1111/j.1755-0998.2008.02090.x

Abstract

We developed 16 novel polymorphic tetranucleotide microsatellite markers for Poecilia parae, a livebearing fish used in evolutionary studies because of its Y-linked colour and size polymorphism. A set of 199 clones was sequenced out of an enriched genomic library, and we achieved an enrichment efficiency of nearly 80%. Primers were designed for 16 pure repeats, and 59 P. parae were screened for polymorphism. Cross-amplification was tested on Poecilia picta and Poecilia reticulata, the guppy. The new microsatellite markers showed an exceptionally high allelic diversity and low stutter formation, proving their suitability for a broad range of applications in these species.