Nuclear formation in a Drosophila cell-free system

Exp Cell Res. 1990 Nov;191(1):64-70. doi: 10.1016/0014-4827(90)90036-a.

Abstract

A cell-free preparation obtained from 0- to 5-h-old Drosophila melanogaster embryos induces chromatin decondensation and nuclear formation from demembranated Xenopus sperm. Newly formed nuclei have a peripheral lamina, a double membrane, and structures resembling pore complexes. Indirect immunofluorescence analyses demonstrate the association of Drosophila lamins and DNA topoisomerase II with newly assembled nuclei.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Cell Nucleus* / enzymology
  • Cell Nucleus* / metabolism
  • Cell Nucleus* / ultrastructure
  • Cell-Free System
  • Chromatin
  • DNA Topoisomerases, Type II / metabolism
  • Drosophila melanogaster
  • Embryo, Nonmammalian
  • Fluorescent Antibody Technique
  • In Vitro Techniques
  • Lamins
  • Male
  • Nuclear Envelope / metabolism
  • Nuclear Envelope / ultrastructure
  • Nuclear Proteins / metabolism
  • Spermatozoa / ultrastructure
  • Xenopus

Substances

  • Antibodies, Monoclonal
  • Chromatin
  • Lamins
  • Nuclear Proteins
  • DNA Topoisomerases, Type II