Abstract
Ten new polyhydroxyolean-12-ene pentacyclic triterpenoidal saponins, named rogchaponins 1-10, were isolated from the methanolic extract of the roots of Camellia sinensis by a series of chromatographic methods (silica gel flash column and C18 MPLC followed by C18 HPLC). Their structures were established by 1D and 2D-NMR techniques along with IR and HR-TOF-MS. Rogchaponins R4 ( 4) and R5 (5) showed inhibitory activities against yeast alcohol dehydrogenase (ADH) with IC (50) values of 16.1 ± 3.2 and 15.4 ± 3.3 µM, respectively. A 4-methylpyrazole positive control exhibited an IC (50) of 2750 ± 50 µM. However, the saponins showed no inhibitory activity against yeast aldehyde dehydrogenase (ALDH).
© Georg Thieme Verlag KG Stuttgart · New York.
MeSH terms
-
Acids / chemistry
-
Alcohol Dehydrogenase / antagonists & inhibitors*
-
Alcohol Dehydrogenase / chemistry
-
Camellia sinensis / chemistry*
-
Chromatography, High Pressure Liquid
-
Enzyme Inhibitors / chemistry
-
Enzyme Inhibitors / isolation & purification
-
Enzyme Inhibitors / pharmacology
-
Hydrolysis
-
Inhibitory Concentration 50
-
Magnetic Resonance Spectroscopy
-
Mass Spectrometry / methods
-
Plant Extracts / chemistry
-
Plant Roots / chemistry*
-
Saccharomyces cerevisiae / enzymology
-
Saponins / chemistry
-
Saponins / isolation & purification*
-
Saponins / pharmacology
-
Triterpenes / chemistry
-
Triterpenes / isolation & purification*
-
Triterpenes / pharmacology
Substances
-
Acids
-
Enzyme Inhibitors
-
Plant Extracts
-
Saponins
-
Triterpenes
-
Alcohol Dehydrogenase