Pulsed-field gel electrophoresis analysis of more than one clinical isolate of Campylobacter spp. from each of 49 patients in New Zealand

J Clin Microbiol. 2012 Feb;50(2):457-9. doi: 10.1128/JCM.05928-11. Epub 2011 Nov 23.

Abstract

Pulsed-field gel electrophoresis (PFGE) analysis demonstrated that while 76% of patients had only one genotype of campylobacter, 10% carried two different but related genotypes (Dice coefficients > 0.78), and 14% carried at least two unrelated genotypes (Dice coefficients < 0.65). This supports the clustering of Campylobacter isolates with similar PFGE patterns, highlights the need to analyze multiple isolates from both sources and patients, and confirms that caution should be exercised before epidemiological links between patients or sources are dismissed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Campylobacter / classification*
  • Campylobacter / genetics
  • Campylobacter / isolation & purification*
  • Campylobacter Infections / microbiology*
  • Cluster Analysis
  • Coinfection / microbiology*
  • Electrophoresis, Gel, Pulsed-Field*
  • Genotype
  • Humans
  • Molecular Epidemiology
  • Molecular Typing*
  • New Zealand