This paper reports the construction of a novel multi-sensitive chemically crosslinked injectable hydrogel with strong mechanical strength by modifying Pluronic F127 responsive against temperature, pH and redox potential. Crosslinked polymer between benzaldehyde grafted Pluronic (P-A) and amine end capped Pluronic having disulfide linkage (P-B) have been synthesized and characterized with 1H NMR spectroscopy and GPC. The hydrogel under physiological conditions significantly altered sol-gel transition behaviors with much lower critical gelation concentrations and temperatures, compared to Pluronic hydrogels. The rheological characterization demonstrated that the moduli of the hydrogels were able to be tuned depending on molecular weight as well as pH, redox and temperature conditions.