Software for precise tracking of cell proliferation

Hiroshi Kurokawa; Hisayori Noda; Mayu Sugiyama; Asako Sakaue-Sawano; Kiyoko Fukami; Atsushi Miyawaki

doi:10.1016/j.bbrc.2011.12.100

Software for precise tracking of cell proliferation

Biochem Biophys Res Commun. 2012 Jan 20;417(3):1080-5. doi: 10.1016/j.bbrc.2011.12.100. Epub 2011 Dec 27.

Authors

Hiroshi Kurokawa¹, Hisayori Noda, Mayu Sugiyama, Asako Sakaue-Sawano, Kiyoko Fukami, Atsushi Miyawaki

Affiliation

¹ Brain Science Institute, RIKEN, Wako-city, Saitama, Japan.

PMID: 22226970
DOI: 10.1016/j.bbrc.2011.12.100

Abstract

We have developed a multi-target cell tracking program TADOR, which we applied to a series of fluorescence images. TADOR is based on an active contour model that is modified in order to be free of the problem of locally optimal solutions, and thus is resistant to signal fluctuation and morphological changes. Due to adoption of backward tracing and addition of user-interactive correction functions, TADOR is used in an off-line and semi-automated mode, but enables precise tracking of cell division. By applying TADOR to the analysis of cultured cells whose nuclei had been fluorescently labeled, we tracked cell division and cell-cycle progression on coverslips over an extended period of time.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Cycle*
Cell Division*
Cell Proliferation*
Cell Tracking / methods*
Fluorescence
Fluorescent Dyes / analysis
HeLa Cells
Humans
Image Processing, Computer-Assisted / methods*
Software*

Substances

Fluorescent Dyes