Abstract
A new chromenoquinoline-based fluorescent off-on thiol probe 2 is reported. In aqueous buffer solutions at physiological pH, the probe exhibited 223-fold enhancement in fluorescence intensity by a Michael addition of cysteine to the maleimide appended to a chromenoquinoline. Cell permeability and live cell imaging of thiols are also demonstrated.
This journal is © The Royal Society of Chemistry 2012
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Benzopyrans / chemistry*
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Cell Line, Tumor
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Cell Membrane Permeability
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Cysteine / chemistry
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Fluorescent Dyes / chemistry*
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Humans
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Maleimides / chemistry
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Microscopy, Fluorescence
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Quantum Theory
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Quinolines / chemistry*
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Sulfhydryl Compounds / chemistry*
Substances
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Benzopyrans
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Fluorescent Dyes
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Maleimides
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Quinolines
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Sulfhydryl Compounds
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maleimide
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quinoline
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Cysteine