Abstract
Lyme disease caused by spirochete Borrelia burgdorferi sensu lato, is a tick-born illness. If the infection is not eliminated by the host immune system and/or antibiotics, it may further disseminate and cause severe chronic complications. The immune response to Borrelia is mediated by phagocytic cells and by Borrelia-specific complement-activating antibodies associated with Th1 cell activation. A new experimental vaccine was constructed using non-lipidized form of recombinant B. burgdorferi s.s. OspC protein was anchored by metallochelating bond onto the surface of nanoliposomes containing novel nonpyrogenic lipophilized norAbuMDP analogues denoted MT05 and MT06. After i.d. immunization, the experimental vaccines surpassed Alum with respect to OspC-specific titers of IgG2a, IgG2b isotypes when MT06 was used and IgG3, IgM isotypes when MT05 was used. Both adjuvants exerted a high adjuvant effect comparable or better than MDP and proved themselves as nonpyrogenic.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylmuramyl-Alanyl-Isoglutamine / chemistry*
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Acetylmuramyl-Alanyl-Isoglutamine / toxicity
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Animals
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Antibodies, Bacterial / blood*
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Antigens, Bacterial / immunology*
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Bacterial Outer Membrane Proteins / immunology*
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Borrelia burgdorferi / immunology*
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Calorimetry, Differential Scanning
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Chelating Agents / chemistry*
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Chelating Agents / toxicity
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Drug Carriers / chemistry*
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Drug Carriers / toxicity
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Electrophoresis, Polyacrylamide Gel
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Enzyme-Linked Immunosorbent Assay
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Female
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Light
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Liposomes
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Lyme Disease Vaccines / administration & dosage
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Lyme Disease Vaccines / immunology*
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Mice
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Mice, Inbred BALB C
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Microscopy, Electron, Transmission
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Nanoparticles / chemistry*
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Nanoparticles / toxicity
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Scattering, Radiation
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Antibodies, Bacterial
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Antigens, Bacterial
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Bacterial Outer Membrane Proteins
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Chelating Agents
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Drug Carriers
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Liposomes
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Lyme Disease Vaccines
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OspC protein
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Acetylmuramyl-Alanyl-Isoglutamine