Abstract
Natural antisense transcripts (NATs) are naturally widespread and have very important functions in regulating expression of their target genes. In this report, we identified a novel NAT, designated mucD-AS, transcribed from the opposite strand ofmucD gene locus, which has a pivotal function in regulating the biosynthesis of alginate. For the biotechnological and medical significance in the study of alginate biosynthesis, we investigated the effect ofmucD-AS on mucD expression and alginate biosynthesis, and confirmed mucD-AS can induce biofilm formation ofP. aeruginosa significantly. Since alginate has a key function in the infection process and acts as virulence factor of P. aeruginosa, we speculate mucD-AS may have a regulatory function in this process.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alginates
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / genetics*
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Base Sequence
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Biofilms / growth & development*
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Blotting, Northern
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Gene Expression Regulation, Bacterial*
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Genes, Bacterial
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Glucuronic Acid / biosynthesis
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Glucuronic Acid / genetics
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Hexuronic Acids
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Molecular Sequence Data
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Pseudomonas aeruginosa / genetics*
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Pseudomonas aeruginosa / metabolism
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Pseudomonas aeruginosa / pathogenicity
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RNA, Antisense / genetics*
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RNA, Antisense / metabolism
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Sigma Factor / biosynthesis
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Sigma Factor / genetics*
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Transcription, Genetic
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Virulence Factors / biosynthesis*
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Virulence Factors / genetics
Substances
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Alginates
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Bacterial Proteins
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Hexuronic Acids
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RNA, Antisense
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Sigma Factor
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Virulence Factors
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algN protein, Pseudomonas aeruginosa
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Glucuronic Acid