Free-radical degradation of high-molar-mass hyaluronan induced by ascorbate plus cupric ions: testing of stobadine and its two derivatives in function as antioxidants

Gen Physiol Biophys. 2012 Mar;31(1):57-64. doi: 10.4149/gpb_2012_006.

Abstract

Stobadine·2HCl and its two hydrophilic derivatives SM1dM9dM10·2HCl and SME1i-ProC2·HCl were tested in the function of antioxidants on hyaluronan (HA) degradation induced by the Weissberger oxidative system [ascorbate plus Cu(II)]. As a primary method, rotational viscometry was applied, where the substance tested was added before or 1 h after the initiation of HA degradation. The most effective scavengers of •OH and peroxy-type radicals were recorded to be stobadine·2HCl and SME1i-ProC2·HCl, respectively. The most effective scavenger, determined by applying the ABTS assay, was stobadine·2HCl.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / chemistry*
  • Ascorbic Acid / chemistry*
  • Carbolines / chemistry*
  • Copper / chemistry*
  • Free Radicals / chemistry
  • Hyaluronic Acid / chemistry*
  • Molecular Weight

Substances

  • Antioxidants
  • Carbolines
  • Free Radicals
  • Copper
  • Hyaluronic Acid
  • Ascorbic Acid
  • dicarbine