Abstract
In this paper, a novel amperometric choline biosensor with immobilization of choline oxidase on electrochemically polymerized polypyrrole-polyvinylsulphonate (PPy-PVS) film has been accomplished via the entrapment technique. The effects of pH and temperature were investigated and optimum parameters were found to be 9.0 and 60 °C, respectively. There are two linear parts in the region between 1.0 × 10 (-7) - 1.0 × 10 (-6)M (R(2) = 0.997) and 1.0 × 10 (-5) - 1.0 × 10 (-3) M (R(2) = 0.986). The storage stability and operation stability of the enzyme electrode were also studied.
MeSH terms
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Alcaligenes / enzymology
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Alcohol Oxidoreductases / chemistry*
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Alcohol Oxidoreductases / metabolism
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Biosensing Techniques / instrumentation
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Biosensing Techniques / methods*
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Calibration
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Choline / analysis*
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Choline / chemistry
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Electrochemistry
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Electrodes
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Enzymes, Immobilized / chemistry*
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Enzymes, Immobilized / metabolism
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Hydrogen-Ion Concentration
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Polymers / chemistry*
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Polyvinyls / chemistry*
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Pyrroles / chemistry*
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Sulfonic Acids / chemistry*
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Temperature
Substances
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Enzymes, Immobilized
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Polymers
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Polyvinyls
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Pyrroles
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Sulfonic Acids
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lyapolate
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polypyrrole
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Alcohol Oxidoreductases
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choline oxidase
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Choline