Fish are the major dietary source of very-long-chain n-3 polyunsaturated fatty acids. We investigated whether cultured fish contain fewer n-3 fatty acids than do wild fish. Fifty-eight trout, 51 eel, and 5 salmon were collected from fishermen and hatcheries throughout Europe, pooled into 23 lots (8 of wild and 15 of cultured fish), and analyzed. The ratio of n-3 to n-6 polyunsaturates was significantly lower in cultured than in wild fish (2 vs 7 in trout, 2 vs 5 in eel, and 6 vs 11 in salmon). Hunted fish are a better source of n-3 polyunsaturates than are cultured fish.