Structure of the gene encoding beta-1,3-glucanase A1 of Bacillus circulans WL-12

N Yahata; T Watanabe; Y Nakamura; Y Yamamoto; S Kamimiya; H Tanaka

doi:10.1016/0378-1119(90)90122-8

Structure of the gene encoding beta-1,3-glucanase A1 of Bacillus circulans WL-12

Gene. 1990 Jan 31;86(1):113-7. doi: 10.1016/0378-1119(90)90122-8.

Authors

N Yahata¹, T Watanabe, Y Nakamura, Y Yamamoto, S Kamimiya, H Tanaka

Affiliation

¹ Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University, Japan.

PMID: 2311931
DOI: 10.1016/0378-1119(90)90122-8

Abstract

The nucleotide sequence of the glcA gene encoding the precursor of extracellular beta-1,3-glucanase (beta Gl) A1, a polysaccharidase produced by Bacillus circulans WL-12, was determined. The putative glcA gene was 2046 bp long, encoding a polypeptide of 682 amino acids (aa). The N-terminal aa sequence of beta Gl produced in Escherichia coli harboring the glcA plasmid was identical to that of beta Gl A1 prepared from the culture fluid of B. circulans WL-12. In both proteins, cleavage of the signal sequence of pre-beta Gl occurred between Ala-38 and Ala-39 of the predicted sequences.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacillus / genetics*
Bacterial Proteins / genetics
Base Sequence
Genes, Bacterial*
Glucan Endo-1,3-beta-D-Glucosidase / genetics*
Glucans / metabolism
Glycoside Hydrolases / genetics*
Molecular Sequence Data
Molecular Weight
beta-Glucans*

Substances

Bacterial Proteins
Glucans
beta-Glucans
beta-1,3-glucan
Glycoside Hydrolases
Glucan Endo-1,3-beta-D-Glucosidase

Associated data

GENBANK/M34503