Development of the Islands of Calleja

Abstract

The Islands of Calleja are aggregations of granule cells located in the basal forebrain of most vertebrate species. These cellular aggregations are typically classified as consisting of a single island, the insula magna located adjacent to the nucleus accumbens, and numerous small islands scattered among the dorsal aspect of the olfactory tubercle. While these structures have been widely described in adult, comparatively little is known about their development. Islands are first identifiable at P2-P4 with formation of the Insula Magna and several small aggregations in the caudolateral aspect of the basal forebrain. The Insula Magna fully forms at approximately P4, with continued formation of the small islands through P10 in a caudal to rostral gradient. Historically, there has been controversy as to whether neurons in the islands are GABAergic, due to limitations in resolving immunolabeling for GABA in the densely packed islands. We investigated the neurochemical identity of island cells by exploiting transgenic reporter mice expressing green fluorescent protein under the control of the GAD65 promoter. This demonstrated that the majority of neurons in the Islands of Calleja are GABAergic, primarily utilizing GAD65. Interestingly, several calcium binding protein expressing interneuron classes are present in the postnatal islands, but disappear with maturation. These findings show that the SVZ derived progenitors that migrate to the Islands of Calleja form different lineages to those destined for the olfactory bulbs, despite generation of both populations at the same age/location in the SVZ.