Isolation and characterization of bacteriophage PM2 from Aeromonas hydrophila

FEMS Microbiol Lett. 1990 Mar 15;56(3):239-44. doi: 10.1016/s0378-1097(05)80047-3.

Abstract

PM2 is an Aeromonas-specific bacteriophage isolated on A. hydrophila strain AH-3. The bacteriophage receptor for this phage was found to be the lipopolysaccharide (LPS), specifically a low-molecular weight LPS fraction (LPS-core oligosaccharides). Mutants resistant to this phage were isolated and found to be devoid of LPS O-antigen and altered in the LPS-core. No other outer-membrane (OM) molecules appeared to be involved in phage binding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aeromonas* / metabolism
  • Bacteriophages / isolation & purification*
  • Bacteriophages / metabolism
  • Bacteriophages / ultrastructure
  • Electrophoresis, Polyacrylamide Gel
  • Lipopolysaccharides / analysis
  • Lipopolysaccharides / metabolism*
  • Microscopy, Electron
  • Mutation
  • Receptors, Virus / analysis
  • Receptors, Virus / metabolism*
  • Temperature
  • Viral Plaque Assay

Substances

  • Lipopolysaccharides
  • Receptors, Virus