Crohn's disease and polymorphism of heat shock protein gene HSP70-2 in the Chinese population

Abstract

Background and aims: Crohn's disease (CD) is a multifactorial disorder with a pivotal role of the genetic component. A single nucleotide polymorphism in heat shock protein 70-2 (HSP70-2) has been shown to be associated with a severe clinical course in CD. The purpose of this study was to identify associations between the HSP70-2 polymorphism and the clinical courses of CD in the Chinese population.

Methods: One hundred patients with CD and 190 healthy individuals were genotyped for the HSP70-2 PstI polymorphism by restriction fragment length polymorphism analysis.

Results: The genotype frequency of the PstI polymorphism did not differ between patients and controls. The A allele was higher in CD patients than in controls (61% vs 52%, P = 0.047, odds ratio [OR] = 1.423, 95% confidence interval [CI]: 1.004-2.015). Furthermore, this polymorphism was higher in the penetrating or fistula surgery of CD patients than in controls (63% vs 52%, P = 0.049, OR = 1.530, 95% CI: 1.001-2.337; Table ). But there was no significant difference between the penetrating or fistula surgery patients and no surgery patients (P = 0.673, OR = 0.883, 95% CI: 0.495-1.574). We used multivariate analysis to determine the effects of genotypes on sex, disease behavior, disease location, and so on. No significant difference was observed between these parameters and genotype.

Conclusion: This study reported that the allele A of PstI polymorphism was the association between CD and HSP70-2 gene in the Chinese population. It was also association between penetrating or fistula surgery of CD and HSP70-2 gene in the Chinese population.