Abstract
The aim of this study was to evaluate the effects of an osteogenic medium supplemented with platelet-derived growth factor (PDGF) BB and osteogenic protein (OP) 1 on the proliferation and differentiation of mesenchymal stem cells (MSCs) in an anorganic bovine cancellous bone scaffold. At day 7, there was a statistically significant increase in the number of cells in the scaffolds in the group treated with the medium supplemented with both PDGF-BB and OP-1 when compared with the control groups. The highest alkaline phosphate levels, at 14 and 21 days, were recorded for the samples in medium supplemented with OP-1 alone reflecting osteogenic differentiation. The results commend OP-1, as well as PDGF-BB, for incorporation into porous mineral scaffolds for vertical ridge augmentation.
MeSH terms
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Alkaline Phosphatase / analysis
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Angiogenesis Inducing Agents / pharmacology*
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Animals
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Becaplermin
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Bone Matrix* / chemistry
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Bone Morphogenetic Protein 7 / pharmacology*
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Cattle
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Cell Adhesion / drug effects
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Cell Count
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Cell Culture Techniques
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Cell Differentiation / drug effects
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Cell Proliferation / drug effects
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Culture Media
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DNA / analysis
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Female
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Goats
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Mesenchymal Stem Cells / drug effects*
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Microscopy, Electron, Scanning
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Minerals / chemistry
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Osteogenesis / drug effects
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Porosity
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Proto-Oncogene Proteins c-sis / pharmacology*
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Radiopharmaceuticals
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Recombinant Proteins
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Technetium Tc 99m Medronate
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Time Factors
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Tissue Engineering
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Tissue Scaffolds* / chemistry
Substances
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Angiogenesis Inducing Agents
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BMP7 protein, human
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Bio-Oss
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Bone Morphogenetic Protein 7
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Culture Media
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Minerals
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Proto-Oncogene Proteins c-sis
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Radiopharmaceuticals
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Recombinant Proteins
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Becaplermin
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DNA
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Alkaline Phosphatase
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Technetium Tc 99m Medronate