When studying online movement adjustments, one of the interesting parameters is their latency. We set out to compare three different methods of determining the latency: the threshold, confidence interval, and extrapolation methods. We simulated sets of movements with different movement times and amplitudes of movement adjustments, all with the same known latency. We applied the three different methods in order to determine when the position, velocity, and acceleration of the adjusted movements started to deviate from the values for unperturbed movements. We did so both for averaged data and for the data of individual trials. We evaluated the methods on the basis of their accuracy and precision, and according to whether the latency was influenced by the intensity of the movement adjustment. The extrapolation method applied to average acceleration data gave the most reliable estimates of latency, according to these criteria.