Abstract
Chasing the active impurity: In the validation of a screening hit it was discovered that a polymeric trace impurity was responsible for the biological activity. Such a side product can be formed with similar compounds. During the investigations it was discovered that the negatively charged macromolecule interacts very efficiently with the protein surface of E. coli RNAP via electrostatic interactions.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carboxylic Acids / chemistry*
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Carboxylic Acids / metabolism
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Chromatography, High Pressure Liquid
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DNA / chemistry
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DNA / metabolism
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DNA-Directed RNA Polymerases / antagonists & inhibitors*
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DNA-Directed RNA Polymerases / isolation & purification
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DNA-Directed RNA Polymerases / metabolism
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Enzyme Inhibitors / chemistry*
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Enzyme Inhibitors / metabolism
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Escherichia coli / enzymology
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Protein Binding
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Protein Structure, Tertiary
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Static Electricity
Substances
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Carboxylic Acids
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Enzyme Inhibitors
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DNA
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DNA-Directed RNA Polymerases