Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease

Genetics. 2013 Aug;194(4):1029-35. doi: 10.1534/genetics.113.152710. Epub 2013 May 24.

Abstract

We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.

Keywords: CRISPR RNA; Cas9; Drosophila; genome engineering; homologous recombination.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CRISPR-Cas Systems*
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Drosophila / genetics*
  • Drosophila Proteins / genetics
  • Endodeoxyribonucleases / genetics
  • Endodeoxyribonucleases / metabolism*
  • Gene Targeting
  • Genetic Engineering / methods*
  • Genome, Insect*
  • Germ-Line Mutation
  • Homologous Recombination

Substances

  • Drosophila Proteins
  • y protein, Drosophila
  • Endodeoxyribonucleases