Abstract
Transthyretin (TTR) is a known carrier protein for thyroxine (T4) and retinol-binding protein in the blood that is primarily synthesized in the liver and choroid plexus of the brain. Herein, we report that the TTR gene is expressed in skeletal muscle tissue and up-regulated during myotube formation in C2C12 cells. TTR silencing (TTRkd) significantly reduced myogenin expression and myotube formation, whereas myogenin silencing (MYOGkd) did not have any effect on TTR gene expression. Both TTRkd and MYOGkd led to a decrease in calcium channel related genes including Cav1.1, STIM1 and Orai1. A significant decrease in intracellular T4 uptake during myogenesis was observed in TTRkd cells. Taken together, the results of this study suggest that TTR initiates myoblast differentiation via affecting expression of the genes involved during early stage of myogenesis and the genes related to calcium channel.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Calcium Channels / metabolism
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Cell Differentiation / genetics
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Cell Differentiation / physiology*
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Cell Line
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Gene Expression / genetics
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Male
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Mice
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Mice, Inbred C57BL
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Muscle Development / genetics*
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Muscle Development / physiology*
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Muscle Fibers, Skeletal / metabolism
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Muscle Fibers, Skeletal / physiology*
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Muscle, Skeletal / metabolism
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Muscle, Skeletal / physiology
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Myoblasts / metabolism
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Myoblasts / physiology*
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Myogenin / genetics
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Myogenin / metabolism
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Prealbumin / genetics*
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Prealbumin / metabolism
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Up-Regulation / genetics
Substances
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Calcium Channels
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Myogenin
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Prealbumin
Grants and funding
This work was supported by a grant from the BioGreen 21 Program (project no. PJ 907099), Rural Development Administration, Republic of Korea. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.