A general method of optimizing automated DNA synthesis to decrease chemical consumption to less than half

Anal Biochem. 1990 May 15;187(1):66-75. doi: 10.1016/0003-2697(90)90418-9.

Abstract

An automated DNA synthesis protocol (FC3) has been optimized to decrease the cost of chemical consumption by two- to threefold. Anion-exchange chromatography at pH 12.5 in a Mono Q (Pharmacia) column was used to analyze the oligonucleotides we synthesized. From these analyses, we formulated the FC3 program and a procedure by which a DNA synthesizer designer or operator can use 18-base-long homopolymers of A, G, C, and T to optimize the reaction times, reagent concentrations, solvent wash conditions, and the many steps in the synthesis cycle.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Automation / economics
  • Automation / methods
  • Base Sequence
  • Chromatography, Ion Exchange
  • DNA / chemical synthesis*
  • Electrophoresis, Polyacrylamide Gel
  • Oligonucleotides / chemical synthesis
  • Oligonucleotides / isolation & purification

Substances

  • Oligonucleotides
  • DNA