Abstract
Tumor necrosis factor (TNF) was cytotoxic at concentrations of 10 to 1000 units/ml to 12 of 14 human tumor cell lines. Synergistic or additive cell killing between TNF and radiation was observed in 7 of 10 tumor cell lines, while independent tumor cell killing by each agent occurred in two tumor cell lines. The maximum synergistic effect was observed when TNF was added 4-12 hr prior to irradiation. This interaction was absent when TNF was added after irradiation. TNF also reduced potentially lethal damage repair in 3 of 5 cell lines tested. Possible mechanisms of interaction of TNF and X rays including induction of hydroxyl radicals and subsequent DNA damage by TNF and radiation are discussed.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Carcinoma, Squamous Cell / drug therapy
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Carcinoma, Squamous Cell / radiotherapy
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Cell Survival / drug effects
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Cell Survival / radiation effects
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Dose-Response Relationship, Drug
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Dose-Response Relationship, Radiation
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Drug Interactions
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Drug Synergism
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Humans
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Recombinant Proteins / pharmacology
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Recombinant Proteins / therapeutic use
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Sarcoma, Experimental / drug therapy
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Sarcoma, Experimental / radiotherapy
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Time Factors
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / radiation effects*
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Tumor Necrosis Factor-alpha / pharmacology*
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Tumor Necrosis Factor-alpha / therapeutic use
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X-Rays
Substances
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Recombinant Proteins
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Tumor Necrosis Factor-alpha