Abstract
The study describes a rapid approach for detection of common enteric bacterial pathogens, which involves partial amplification of the 16S rRNA gene by PCR using a colony from selective medium followed by restriction enzyme (RE) digestion using the EcoRI, HindIII and SalI enzymes. On the basis of RE digestion analysis different genera namely, Escherichia, Salmonella, Shigella, Vibrio, Campylobacter, Arcobacter, Yesinia and Listeria were differentiated.
Keywords:
16S rRNA; Diarrhea; Enteric bacterial pathogen; RE digestion.
© 2013.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteria / classification
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Bacteria / genetics
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Bacteria / isolation & purification*
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Bacteriological Techniques / methods*
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DNA Restriction Enzymes / genetics
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DNA Restriction Enzymes / metabolism
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DNA, Bacterial / genetics
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DNA, Bacterial / metabolism
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Gastrointestinal Diseases / diagnosis*
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Gastrointestinal Diseases / microbiology
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Gram-Negative Bacterial Infections / diagnosis
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Gram-Negative Bacterial Infections / microbiology
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Gram-Positive Bacterial Infections / diagnosis
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Gram-Positive Bacterial Infections / microbiology
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Molecular Diagnostic Techniques / methods*
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Polymerase Chain Reaction / methods*
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Polymorphism, Restriction Fragment Length*
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RNA, Ribosomal, 16S / genetics
Substances
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DNA, Bacterial
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RNA, Ribosomal, 16S
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DNA Restriction Enzymes