Characterization of polyphosphate glucokinase SCO5059 from Streptomyces coelicolor A3(2)

Biosci Biotechnol Biochem. 2013;77(11):2322-4. doi: 10.1271/bbb.130498. Epub 2013 Nov 7.

Abstract

SCO5059, encoded in Streptomyces coelicolor A3(2), was identified as a polyphosphate glucokinase. The Km values of SCO5059 for glucose and polyphosphate (poly(P)6) were estimated to be 12 and 4 µM, respectively, and the kcat value was 0.3 s(-1) at pH 7.7 at 28 °C. SCO5059 homologs are highly conserved among Streptomyces, and can work as polyphosphate glucokinase as well.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression
  • Glucose / metabolism
  • Hydrogen-Ion Concentration
  • Kinetics
  • Phosphotransferases / genetics
  • Phosphotransferases / metabolism*
  • Polyphosphates / metabolism
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Streptomyces coelicolor / enzymology*
  • Streptomyces coelicolor / genetics

Substances

  • Bacterial Proteins
  • Polyphosphates
  • Recombinant Proteins
  • Phosphotransferases
  • polyphosphate-glucose phosphotransferase
  • Glucose