Abstract
SCO5059, encoded in Streptomyces coelicolor A3(2), was identified as a polyphosphate glucokinase. The Km values of SCO5059 for glucose and polyphosphate (poly(P)6) were estimated to be 12 and 4 µM, respectively, and the kcat value was 0.3 s(-1) at pH 7.7 at 28 °C. SCO5059 homologs are highly conserved among Streptomyces, and can work as polyphosphate glucokinase as well.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Gene Expression
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Glucose / metabolism
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Hydrogen-Ion Concentration
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Kinetics
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Phosphotransferases / genetics
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Phosphotransferases / metabolism*
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Polyphosphates / metabolism
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Streptomyces coelicolor / enzymology*
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Streptomyces coelicolor / genetics
Substances
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Bacterial Proteins
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Polyphosphates
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Recombinant Proteins
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Phosphotransferases
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polyphosphate-glucose phosphotransferase
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Glucose